| INGREDIENT | 500 mL bottle |
|---|
| Total volume of media | 500 mL |
|---|
| EDTA (dihydrate) | 93 g |
| Distilled water | 500 mL |
| 0.5M NaOH | ~50mL |
- Prepare a beaker with a stir bar
- Pour ~300 mL of water into the beaker
- Calibrate the pH probe and insert it into the beaker, ensure the probe is above the stir bar so it does not hit the probe
- Add 93 g EDTA (dihydrate)
- Add NaOH to adjust the pH until it hits a pH of 8.0
a. ~10 g pellets or ~50 mL of 5 M NaOH
b. The EDTA takes a while to dissolve, you can use a low heat to help
Add water to 500 mL
Store at 4 C in a nonreactive plastic (HDPE/2, LDPE/4, PP/5)
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Orignal Recipe by: E. Duan Date: 30 May 25
Recipe Updated by: ___ Date: ___
Updated on: June 3rd, 2025