Combinational Enhancer Solution (CES) used to improve the efficiency of PCR. Add 5 ul for 25 ul PCR reaction and 10 ul for 50 ul PCR reaction.
INGREDIENT | 1 mL vial |
---|---|
Total volume of media | 1 mL |
2.7 M* betaine (B2629-50G Sigma, MW 117.15) | 540 µL |
6.7 mM* didithiothreitol (DTT, Cleland's reagent) (43819-1G Sigma, MW 154.25) | 200 µL |
55 ug/mL* BSA (albumin bovine serum fraction, A3059-50G from Sigma) | 1 µL |
6.7% (v/v)* DMSO | 67 µL |
Distilled water | 192 µL |
* Final concentration in the CES
Method for making 5xCES:
Reference: Ralser, M., R. Querfurth, H. Warnatz, H. Lehrach, M. Yaspo, S. Krobitsch. 2006. An efficient and economic enhancer mix for PCR. Biochemical and Biophysical Research Communications 347: 747–751.
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Original Recipe by: J. Gallie Date: 20 Sept 10
Recipe Updated by: ___ Date: ___