Plates used for double cross over screening for pkoV allelic replacement.
| INGREDIENT | 1000 mL Flask | 2000 mL flask |
|---|
| Total volume of media | 500 mL | 1000 mL |
|---|
| Tryptone | 5 g | 10 g |
| Yeast Extract | 2.5 g | 5 g |
| Sucrose | 25 g | 50 g |
| Agar | 7.5 g | 15 g |
| Distilled water | 500 mL | 1000 mL |
- Prepare media in desired volumes.
- Carefully add a stir bar to flask and mix ingredients. Leave stir bar in while autoclaving.
- Autoclave on a liquid load for at least 45 minutes.
- After autoclaving, immediately pour plates. Note: Plates should be poured immediatetly although agar can temporarily be stored in the 55°C oven. This should be avoided if possible due to potential bacterial contamination.
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Orignal Recipe by: H. Lindsey Date: 24 July 12
Recipe Updated by: ___ Date: ___