MX (MA, MM, MG) Plates

INGREDIENT	500 mL flask	500 mL flask	1000 mL flask
Total volume of media	500 mL	1000 mL	2000 mL
Potassium Phosphate Dibasic	3.5 g	7 g	10.5 g
Potassium Phosphate Monobasic	1 g	2 g	3 g
Ammonium Sulfate	0.5 g	1 g	1.5 g
Sodium Citrate	0.25 g	0.5 g	0.75 g
Distilled water	150 mL	300 mL	500 mL

INGREDIENT	1000 mL flask	1000 mL flask	2000 mL flask
Total volume of media	500 mL	1000 mL	2000 mL
Agar	8 g	16 g	24 g
Distilled water	250 mL	500 mL	800 mL

INGREDIENT	250 mL flask	250 mL flask	250 mL flask
Total volume of media	500 mL	1000 mL	2000 mL
Sugar*	2 g	4 g	6 g
Distilled water	100 mL	200 mL	200 mL

*Can be Arabinose (MA), Maltose (MM) or Glucose (MG).

Prepare media in desired volumes.
Carefully add a stir bar to flask and mix ingredients. Leave stir bar in while autoclaving.
Autoclave on a liquid load for at least 45 minutes.
After autoclaving, let cool to about 55°C, add the sugar and salts flasks to the agar flask, as well as 1mL of 10% MgSO₄ and 1mL of 0.2% Thiamine per liter of media and pour plates. Note: Plates should be poured immediately although agar can temporarily be stored in the 55°C oven. This should be avoided if possible due to potential bacterial contamination.

Orignal Recipe by: C. Eshelman Date: 23 July 12

Recipe Updated by: ___ Date: ___

Public: MXPlates