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Public: MinimalMediaBroth

INGREDIENT	500 mL bottle	1000 mL bottle	2000 mL bottle
Total volume of media	400 mL	800 mL	1800 mL
Potassium Phosphate Dibasic	2.8 g	5.6 g	12.6 g
Potassium Phosphate Monobasic	0.8 g	1.6 g	3.6 g
Ammonium Sulfate	0.4 g	0.8 g	1.8 g
Sodium Citrate	0.2 g	0.4 g	0.9 g
Distilled water	400 mL	800 mL	1800 mL

Label appropriate sized bottle with tabbed colored tape, including the following information: initials, date, type of media (Minimal Media), and boxes that can be checked for the ingredients that you will add after autoclaving (Sugar, Magnesium Sulfate, and Thiamine).
Prepare media in desired volumes.
Autoclave on a liquid load, for at least 45 minutes.
After autoclaving, let cool to room temperature and add the following stock solutions.

INGREDIENT	400 mL media	800 mL media	1800 mL media
Sterile 10% Sugar Solution	4 mL	8 mL	18 mL
Magnesium Sulfate Solution	0.4 mL	0.8 mL	1.8 mL
Sterile 0.2% Thiamine Solution	0.4 mL	0.8 mL	1.8 mL

Mix ingredients and store at room temperature.

Back to Liquid Media

Original Recipe by: C. Eshelman Date: 26 July 12

Recipe Updated by: ___ Date: ___

Retrieved from http://kerrlab.org/Public/MinimalMediaBroth

Page last modified on September 13, 2012, at 10:24 PM