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Media used for double cross over screening for pkoV allelic replacement.

INGREDIENT	500 mL bottle	1000 mL bottle	2000 mL bottle
Total volume of media	400 mL	800 mL	1800 mL
Tryptone	4 g	8 g	18 g
Yeast Extract	2 g	4 g	9 g
Distilled water	400 mL	800 mL	1800 mL

Prepare media in desired volumes.
Carefully add a stir bar to bottle and mix ingredients. Once mixed, remove stir bar using magnetic stick.
Autoclave on a liquid load for at least 45 minutes.

Orignal Recipe by: H. Lindsey Date: 21 August 12

Recipe Updated by: ___ Date: ___

Public: NoSaltLBBroth