2-3-5-triphenyl tetrazolium chloride (TTC) is a dye which turns red when reduced. Bacterial colonies that are expressing a certain biochemical activity can be identified by visual examination because they have a unique color on media containing a particular dye. In most cases the dye changes color when it is hydrolysed, or acts as a pH or redox indicator. TA (Tetrazolium Arabinose) are the most common plates used in this laboratory. We use TA agar for distinguishing Ara- and Ara+ strains in most competition assays. Colonies of Ara- strains typically appear red on TA agar, while those of Ara+ strains appear white/pink.
INGREDIENT | 1000 mL Flask | 2000 mL flask | 2000 mL flask |
---|---|---|---|
Total volume of media | 500 mL | 1000 mL | 1500 mL flask |
Tryptone | 5 g | 10 g | 15 g |
Yeast Extract | 0.5 g | 1 g | 1.5 g |
Sodium Chloride | 2.5 g | 5 g | 7.5 g |
Agar | 8 g | 16 g | 24 g |
Distilled water | 400 mL | 800 mL | 1200 mL |
INGREDIENT | 250 mL Flask | 250 mL flask | 500 mL flask |
---|---|---|---|
Total volume of media | 500 mL | 1000 mL | 1500 mL flask |
Sugar* | 5 g | 10 g | 15 g |
Distilled water | 100 mL | 200 mL | 300 mL |
*Sugar can be Arabinose (TA), Maltose (TM) or Glucose (TG).
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Original Recipe by: C. Eshelman Date: 26 July 12
Recipe Updated by: ___ Date: ___