LB Broth

INGREDIENT500 mL bottle1000 mL bottle2000 mL bottle
Total volume of media400 mL800 mL1800 mL
Tryptone4 g8 g18 g
Yeast Extract2 g4 g9 g
Sodium Chloride4 g8 g18 g
Distilled water400 mL800 mL1800 mL
  1. Prepare media in desired volumes.
  2. Carefully add a stir bar to bottle and mix ingredients. Once mixed, remove stir bar using magnetic stick.
  3. Autoclave on a liquid load for at least 45 minutes.

Back to Liquid Media

Orignal Recipe by: C. Eshelman Date: 24 July 12

Recipe Updated by: ___ Date: ___

Categories: Recipes