Making Antibiotic Stock
Purpose
Directions for making antibiotic stocks. Recipes adapted from Miller 1992: A Short Course in Bacterial Genetics
Antibiotic Safety Information
Almost all antibiotics are toxic to humans. Wear gloves and a lab coat, work in fume hood, and be careful not to inhale/ingest or have skin contact with antibiotics.
| Antibiotic | Flammable? | Toxic? | Light-sensitive? | Temperature sensitive? |
|---|---|---|---|---|
| Rifampicin | yes | yes | yes | yes, store below 23 C |
| Tetracycine | yes | yes | yes | yes, store below 23 C |
| Streptomycin | yes | yes | no | yes, store below 23 C |
| Kanamycin | yes | yes | no | yes, store below 23 C |
| Ampicillin | yes | yes | no | yes, store below 23 C |
| Chloramphenicol | yes | yes | no | yes, store below 23 C |
| Nalidixic Acid | yes | yes | no | yes, store below 23 C |
Rifampicin: See the following page for Rifampicin, which requires special instructions?.
Tetracycline (15 mg/ml stock solution) MSDS
Tetracycline is light sensitive and flammable. Make stock in fume hood. Weigh out 15 mg of tetracycline. Dissolve in 0.5 ml of sterile dH2O and 0.5 ml of ethanol under the fume hood. Filter sterilize stock into a sterile test tube wrapped in foil. Add 1 ml stock solution directly to 1 liter of cooling agar for a final plate concentration of 15 µg/ml.