TBE Electrophoresis Buffer
10x TBE Buffer Stock
| INGREDIENT | 1000 mL bottle |
|---|---|
| Total volume of media | 1000 mL |
| Tris base | 108 g |
| Boric acid | 55 g |
| 0.5M EDTA, pH = 8.0 | 40 mL |
| Distilled water | up to 1000mL |
- Add dry ingredients and a stir bar to the bottle along with around half the water.
- Put on the stir plate and add the EDTA slowly.
- After the dry ingredients go into solution (this will take a bit) add dH2O up to 1000mL.
- Dilute 100mL of 10x buffer in 900mL of dH2O for 1x buffer solution. This concentration will be used to make and run electrophoresis gels.
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Original Recipe by: C. Eshelman Date: 13 Sept 12
Recipe Updated by: ___ Date: ___
Categories: Recipes