Minimal Media Broth

INGREDIENT500 mL bottle1000 mL bottle2000 mL bottle
Total volume of media400 mL800 mL1800 mL
Potassium Phosphate Dibasic2.8 g5.6 g12.6 g
Potassium Phosphate Monobasic0.8 g1.6 g3.6 g
Ammonium Sulfate0.4 g0.8 g1.8 g
Sodium Citrate0.2 g0.4 g0.9 g
Distilled water400 mL800 mL1800 mL
  1. Label appropriate sized bottle with tabbed colored tape, including the following information: initials, date, type of media (Minimal Media), and boxes that can be checked for the ingredients that you will add after autoclaving (Sugar, Magnesium Sulfate, and Thiamine).
  2. Prepare media in desired volumes.
  3. Autoclave on a liquid load, for at least 45 minutes.
  4. After autoclaving, let cool to room temperature and add the following stock solutions.
INGREDIENT400 mL media800 mL media1800 mL media
Sterile 10% Sugar Solution4 mL8 mL18 mL
Magnesium Sulfate Solution0.4 mL0.8 mL1.8 mL
Sterile 0.2% Thiamine Solution0.4 mL0.8 mL1.8 mL
  • Mix ingredients and store at room temperature.

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Original Recipe by: C. Eshelman Date: 26 July 12

Recipe Updated by: ___ Date: ___

Categories: Recipes