Minimal Media Broth
| INGREDIENT | 500 mL bottle | 1000 mL bottle | 2000 mL bottle |
|---|---|---|---|
| Total volume of media | 400 mL | 800 mL | 1800 mL |
| Potassium Phosphate Dibasic | 2.8 g | 5.6 g | 12.6 g |
| Potassium Phosphate Monobasic | 0.8 g | 1.6 g | 3.6 g |
| Ammonium Sulfate | 0.4 g | 0.8 g | 1.8 g |
| Sodium Citrate | 0.2 g | 0.4 g | 0.9 g |
| Distilled water | 400 mL | 800 mL | 1800 mL |
- Label appropriate sized bottle with tabbed colored tape, including the following information: initials, date, type of media (Minimal Media), and boxes that can be checked for the ingredients that you will add after autoclaving (Sugar, Magnesium Sulfate, and Thiamine).
- Prepare media in desired volumes.
- Autoclave on a liquid load, for at least 45 minutes.
- After autoclaving, let cool to room temperature and add the following stock solutions.
| INGREDIENT | 400 mL media | 800 mL media | 1800 mL media |
|---|---|---|---|
| Sterile 10% Sugar Solution | 4 mL | 8 mL | 18 mL |
| Magnesium Sulfate Solution | 0.4 mL | 0.8 mL | 1.8 mL |
| Sterile 0.2% Thiamine Solution | 0.4 mL | 0.8 mL | 1.8 mL |
- Mix ingredients and store at room temperature.
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Original Recipe by: C. Eshelman Date: 26 July 12
Recipe Updated by: ___ Date: ___
Categories: Recipes