No Salt LB Broth

Media used for double cross over screening for pkoV allelic replacement.

INGREDIENT500 mL bottle1000 mL bottle2000 mL bottle
Total volume of media400 mL800 mL1800 mL
Tryptone4 g8 g18 g
Yeast Extract2 g4 g9 g
Distilled water400 mL800 mL1800 mL
  1. Prepare media in desired volumes.
  2. Carefully add a stir bar to bottle and mix ingredients. Once mixed, remove stir bar using magnetic stick.
  3. Autoclave on a liquid load for at least 45 minutes.

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Orignal Recipe by: H. Lindsey Date: 21 August 12

Recipe Updated by: ___ Date: ___

Categories: Recipes