Saline Buffer
| INGREDIENT | 500 mL bottle | 1000 mL bottle | 2000 mL bottle |
|---|---|---|---|
| Total volume of media | 400 mL | 800 mL | 1800 mL |
| Sodium Chloride | 3.4 g | 6.8 g | 17.2 g |
| Distilled water | 400 mL | 800 mL | 1800 mL |
- Prepare media in desired volumes.
- Carefully add a stir bar to flask and mix ingredients. Remove stir bar before autoclaving.
- Autoclave on a liquid load for at least 45 minutes.
- Store at room temperature.
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Original Recipe by: C. Eshelman Date: 26 July 12
Recipe Updated by: ___ Date: ___
Categories: Recipes