MX Plates

MX (MA, MM, MG) Plates

MX Salts

INGREDIENT500 mL flask500 mL flask1000 mL flask
Total volume of media500 mL1000 mL2000 mL
Potassium Phosphate Dibasic3.5 g7 g10.5 g
Potassium Phosphate Monobasic1 g2 g3 g
Ammonium Sulfate0.5 g1 g1.5 g
Sodium Citrate0.25 g0.5 g0.75 g
Distilled water150 mL300 mL500 mL

MX Agar

INGREDIENT1000 mL flask1000 mL flask2000 mL flask
Total volume of media500 mL1000 mL2000 mL
Agar8 g16 g24 g
Distilled water250 mL500 mL800 mL

MX Sugar

INGREDIENT250 mL flask250 mL flask250 mL flask
Total volume of media500 mL1000 mL2000 mL
Sugar*2 g4 g6 g
Distilled water100 mL200 mL200 mL

*Can be Arabinose (MA), Maltose (MM) or Glucose (MG).

  1. Prepare media in desired volumes.
  2. Carefully add a stir bar to flask and mix ingredients. Leave stir bar in while autoclaving.
  3. Autoclave on a liquid load for at least 45 minutes.
  4. After autoclaving, let cool to about 55°C, add the sugar and salts flasks to the agar flask, as well as 1mL of 10% MgSO4 and 1mL of 0.2% Thiamine per liter of media and pour plates. Note: Plates should be poured immediately although agar can temporarily be stored in the 55°C oven. This should be avoided if possible due to potential bacterial contamination.

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Orignal Recipe by: C. Eshelman Date: 23 July 12

Recipe Updated by: ___ Date: ___

Categories: Recipes