Rif Ramp Protocols
Most protocols are described in detail in the manuscript, however we provide a more detailed version of our genetic engineering protocol here:
The following protocols are referenced in the Primer Mediated Site-Directed Mutagenesis and pkoV Mediated Allelic Replacement protocol:
For all of our fitness assays, microtiter plates containing 200uL of Minimal Glucose media were inoculated with scrapings from the freezer and recovered at 37°C for 24 hours or for 48 hours in the case of a couple very slow growing strains.
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